Reduction in mitochondrial membrane peroxidizability index…
621
Figure 1.-‐ Mitochondrial ETC complexes, MnSOD, AIF, SIRT3 and SIRT5 from control and
atenolol treated Wistar rats.
A, left: Representative immunoblots showing relative levels of
mitochondrial complex subunits from complex I to IV of heart mitochondria from control and
atenolol treated Wistar rats; right: The relative values were obtained by densitometric analyses.
The intensity of each product was related to the control protein porin. B, left: Representative
immunoblots showing relative levels of regulatory factors of mitochondrial biology of heart
mitochondria from control and atenolol treated wistar rats; right: The relative values were
obtained by densitometric analyses. The intensity of each product was related to the control
protein porin. Values are means ± SEM from 6-‐7 diferent samples per group. Asterisks represent
significant differences compared to the control group: * P<0.05.
Figure 2.-‐ Protein oxidation, glycoxidation and lipoxidation markers of heart mitochondria
from control and atenolol treated Wistar rats.
Values are means ± SEM from 8 different samples
and are expressed as percentage from control group. Control values: 5276.91±238.38 (Glutamic
SemiAldehyde, GSA); 229.67±4.31 (AASA, AminoAdipic SemiAldehyde, AASA); 605.79±23.36
(CarboxyEthyl-‐Lysine,
CEL); 1558.30±62.06 (CarboxyMethyl-‐Lysine,
CML); 584.47±51.77
(MalonDiAldehyde-‐Lysine, MDAL). Units: µmol/mol lysine. Asterisks represent significant
differences between control and atenolol rats: *** P<0.001.
