An. Real. Acad. Farm. vol 79 nº 4 2013 - page 107

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The fatty acid composition of heart mitochondria lipids and derived indexes
were also measured (Table 4). The fatty acids 18:0, 20:4n-­‐6, 22:0, 22:4n-­‐6, 22:5n-­‐
6, 22:5n-­‐3, 22:6n-­‐3 and 24:0 were significantly lower and the fatty acid 18:2n-­‐6
was significantly higher in the atenolol group. Due to these changes the acyl chain
length decreased (ACL; 0.66% total decrease), the PUFAn-­‐3 strongly decreased
(27.61% total decrease) and the PUFAn-­‐6 increased (12.31 % total increase). The
final result was a strong decrease in the peroxidizability index (PI; 15.20% total
decrease) and a non-­‐significant trend to decrease the total number of double
bonds (6.94% total decrease in the double bond index, DBI) in the atenolol group
in relation to the control group (P<0.01 in PI; Figure 3).
The estimation of the desaturase and elongase activities is shown in Tables
5 and 6. The desaturase activity Δ9 (n-­‐9) was higher, while Δ5 (n-­‐6) was lower in
the atenolol group. Integrated n-­‐6 and n-­‐3 desaturation and elongation activities
were also lower in the atenolol group. The elongase activities ELOVL 1/3, ELOVL 5
(n-­‐6), ELOVL 2/5 (n-­‐6) and ELOVL 2/5 (n-­‐3) were significantly lower in the
atenolol group.
Figure 3.-­‐ PI and DBI (fatty acid unsaturation degree) and total PUFAn-­‐6 and PUFAn-­‐3 of
heart mitochondria from control and atenolol treated Wistar rats.
PUFA= polyunsaturated
fatty acid; DBI=double bond index; PI= Peroxidizability index. Abbreviations and calculations of
fatty acid classes and indexes are explained in the Materials and Methods section. Control values:
116.22±5.49 (PI); 131.93±4.70 (DBI); and 7.10±0.39 (for PUFAn-­‐3) and 25.01±0.97 (for PUFAn-­‐6),
both expressed as mol %. Values are means ± SEM from 8 different samples per group. Asterisks
represent significant differences compared to the control group: * P<0.05; ** P<0.01; ***P<0.001.
1...,97,98,99,100,101,102,103,104,105,106 108,109,110,111,112,113,114,115,116,117,...190
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